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Mycophenolate mofetil inhibits human Tenon fibroblast proliferation by guanosine depletion

Department of Ophthalmology, University of Essen, Hufelandstrasse 55, 45122 Essen, Germany

Correspondence to:
Correspondence to:
Carsten Heinz
MD, Department of Ophthalmology, University Essen, Hufelandstrasse- 55, 45122Essen, Germany; carsten.heinz{at}gmx.net

ABSTRACT
Backgroundaim: Mycophenolate mofetil could be a useful antiproliferative drug in glaucoma filtering surgery. In this study the authors set out to investigate whether growth inhibition of mycophenolate mofetil on human Tenon fibroblasts is mediated by guanosine depletion.

Methods: Cultured human Tenon fibroblasts were incubated in various concentrations of mycophenolate mofetil with and without supplementation of guanosine.

Results: Growth inhibition was concentration dependent. The effect could be completely antagonised by guanosine supplement.

Conclusion: Human Tenon fibroblasts depend on the de novo synthesis pathway of guanosine. No sufficient salvage pathway from purine degradation appears to exist.

Keywords: mycophenolate mofetil; fibroblast proliferation; guanosine depletion

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