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Published Online First: 7 February 2006. doi:10.1136/bjo.2005.086769 British Journal of Ophthalmology 2006;90:674-678 Copyright © 2006 by the BMJ Publishing Group Ltd.
Human glaucoma and neural degeneration in intracranial optic nerve, lateral geniculate nucleus, and visual cortexN Gupta1,2,3,4, L-C Ang3, L Noël de Tilly5, L Bidaisee4, Y H Yücel2,3,4,*
1 Glaucoma and Nerve Protection Unit, St Michael’s Hospital, Toronto, Canada
Correspondence to:
Accepted 1 February 2006
Abbreviations: GHT, glaucoma hemifield test; H&E/LFB, haematoxylin and eosin/luxol fast blue; IOP, intraocular pressure; LGN, lateral geniculate nucleus; MD, mean deviation; MRI, magnetic resonance imaging; PSD, pattern standard deviation; RGC, retinal ganglion cell Keywords: human glaucoma; pathology; visual field; optic nerve; lateral geniculate nucleus; visual cortex The pathology of glaucoma has been extensively studied at the level of the retina and optic nerve head. Recently, investigations in experimental monkey glaucoma show glaucomatous injury also at the level of the lateral geniculate nucleus (LGN), and visual cortex.1–6 Here we report the first clinicopathological case of human glaucoma, demonstrating degenerative changes in the brain involving the intracranial optic nerves, LGN, and visual cortex.
On examination, best corrected visual acuity for mild hyperopia was 20/20 in each eye and pupils were equally reactive to light. Intraocular pressure (IOP) was 16 mm Hg in both eyes. Angles were wide open with no evidence of pseudoexfoliation or pigment dispersion. Mild nuclear sclerosis was evident in each eye. Optic nerve heads showed large cup to disc ratio of 0.9, with deep excavation and loss of the inferotemporal rim in each eye. No disc haemorrhage was noted. Humphrey 24-2 visual fields showed superior field loss in each eye (fig 1
One year later, the patient died of acute viral myocarditis, and the optic nerves and the brain were examined at autopsy. Four pairs of optic nerves and three brains of age matched patients without neurological or eye disease, were studied as controls (table 1  ).
This study was performed with institutional ethics review board approval. Brain sectioning of the left cerebral hemispheres and coronal slices showed no infarct, intracranial mass, haemorrhage, or diffuse cortical atrophy. Tissue sections were sampled from orbito-frontal, frontal, sensorimotor, temporal, visual, cingulate, superior and inferior parietal cortices, basal ganglia, thalamus with LGN and hippocampus, brainstem including midbrain and medulla, and cerebellum. Haematoxylin and eosin/luxol fast blue (H&E/LFB) stained sections, Bielschowsky silver stained sections, and ß amyloid and tau protein immunostained sections showed no evidence of cerebrovascular, Alzheimer’s, or Parkinson’s disease. The optic nerves and the right cerebral hemisphere posterior to chiasm were cryoprotected, frozen, and serially cut with a sliding microtome (SM3400, Leica, Nussloch, Germany) as previously described.1 Every 15th section was Nissl stained with cresyl violet.
Lateral geniculate nucleus degeneration in human glaucoma The right cerebral hemisphere containing the LGN of the patient with glaucoma and that of a control brain were evaluated with magnetic resonance imaging (MRI). Serial coronal MRI inversion recovery scans showed high signal intensity of the LGN. The largest LGN cross section in the glaucoma patient appeared smaller than the age matched control (table 1  , last row) LGN (fig 3).
LGN sections stained with Nissl showed six layers from ventral to dorsal, magnocellular layers 1 and 2, and parvocellular layers 3–6. Compared to the control (fig 3C ), there was striking overall LGN shrinkage in glaucoma (fig 3D). Right LGN volumes for glaucoma and three age matched controls were calculated using serial frozen sections (50 µm) by point counting (Cavalieri principle and Neurozoom software (Human Brain Project, La Jolla, CA, USA)).1 The LGN volume in glaucoma (69 mm3) was reduced compared to controls (mean 95.9 mm3 (SD 13.5)) (fig 3E).
Magnocellular layers
Cross sectional area measurements Morphometric studies were performed in the right LGN magnocellular layers for the patient with glaucoma and the three age matched controls. Neuron cross sectional area was measured in the right posterior-lateral LGN with unbiased randomised stereological methodology using a nucleator probe (Stereoinvestigator, Microbrightfield Inc, Williston, VT, USA). Contours were drawn around the magnocellular layers in the two most posterior sections at low power (x1.25). Cross sectional area measurements were taken at high power using an oil immersion objective (x60) (table 2 ).
The radius of the neurons was calculated as the square root of the cross sectional area divided by  . The radius of magnocellular neurons in the index glaucoma case was smaller than each of the age matched controls as seen in box plots in figure 4C. The mean cross sectional area of magnocellular neurons in this index glaucoma case was significantly smaller than each of the age matched controls (p<0.0001; two tailed t test).
Parvocellular layers
Cross sectional area measurements Morphometric studies were performed in the right LGN parvocellular layers in the index glaucoma case and three age matched controls. The cross sectional area of neurons in the right posterior-lateral LGN were measured as described above (table 2 ). The radius of parvocellular neurons in this index glaucoma case was smaller than each of the age matched controls (fig 5C), but this difference was statistically significant with only one of the controls (p<0.0001; two tailed t test).
Visual cortex changes in human glaucoma
DISCUSSION This clinicopathological case of advanced human glaucoma and visual field loss, demonstrates the presence of central neural degeneration at multiple levels within the visual system. The neural pathology observed within the intracranial optic nerves, posterior-lateral part of the LGN, and the visual cortex below the calcarine sulcus, correlates with clinically apparent inferior rim loss of the optic nerve head and the superior visual field defect. The smaller size of the LGN detected by MRI suggests a possible role for refined neuroradiological assessment of central visual pathways in glaucoma patients. Reduced cell size was observed in LGN neurons in this case of human glaucoma, and has also been observed in experimental primate glaucoma.2,4,6 Neuron loss in the LGN has been shown in the experimental primate model of glaucoma, with neuron numbers derived from three dimensional neuronal density multiplied by LGN layer volume.1,2 In human glaucoma, however, the data on neuron loss in LGN are limited to a single study10 in which LGN volume decrease was not taken into account.1,11 Selective neuron loss in magnocellular layers concluded by the authors was based on two dimensional density measurements,10 and these two dimensional calculations may have overestimated neuron number and underestimated neuron loss in parvocellular layers. Thus, we used three dimensional methodology of the entire LGN in this study to overcome some of these biases.12 Similar studies on a larger number of neuropathological materials are required to accurately assess neuron loss in the LGN in human glaucoma. A single IOP reading of 16 mm Hg was available in this patient before glaucoma therapy. Thus, while it is possible that this patient had low tension glaucoma, high tension glaucoma cannot be excluded on the basis of this measurement.13 More studies are needed to refine our understanding of central nervous system changes in high and low pressure glaucomas. On average, there is one retinal ganglion cell (RGC) for every LGN neuron14 with retinotopic representation of the visual field.9,15 As much as 50% RGC loss may occur before detection of visual field loss in human glaucoma.16 In the presence of 50% visual field loss as in this case of human glaucoma, neural degeneration is evident at multiple vision stations in the brain including the visual cortex. A multidisciplinary approach is needed to better understand glaucomatous pathology in the central nervous system at various disease stages.17 Treatment strategies to prevent disease progression in glaucoma should also consider central neural degeneration beyond the retina and optic nerve head.
Competing interest statement: there are no competing interests to be declared.
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